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Lamp2 gfp

Tīmeklis2005. gada 15. nov. · High levels of LAMP1-GFP overexpression elicited aberrant aggregation of late endocytic organelles, a phenomenon that probably involved formation of anti-parallel dimers of LAMP1-GFP as it was not observed in cells expressing comparable levels of a non-dimerizing mutant variant, LAMP1-mGFP. To … Tīmeklis2009. gada 13. apr. · We labeled cryosections from β′-COP–depleted cells with anti-LAMP2 antibodies and anti-GFP to detect GFP-LC3 . GFP-LC3 was detected on the …

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Tīmeklis2024. gada 10. maijs · LAMP2 is a lysosomal membrane protein that regulates macroautophagy and chaperone-mediated autophagy (CMA) [ 17 ], promoting the … Lysosome-associated membrane protein 2 (LAMP2), also known as CD107b (Cluster of Differentiation 107b) and Mac-3, is a human gene. Its protein, LAMP2, is one of the lysosome-associated membrane glycoproteins. The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. … freeload shortclip artbbs https://familie-ramm.org

The Golgi-associated retrograde protein (GARP) complex plays an ...

TīmeklisKR20240031832A KR1020247044336A KR20247044336A KR20240031832A KR 20240031832 A KR20240031832 A KR 20240031832A KR 1020247044336 A KR1020247044336 A KR 1020247044336A KR 20247044336 A KR20247044336 A KR 20247044336A KR 20240031832 A KR20240031832 A KR 20240031832A Authority … TīmeklisThat Utilize Tg(hsp70l:lamp2-EGFP) Construct Fish Affected Genomic Regions Phenotype Gene Expression; pd1064Tg; pd1082Tg: Fig. 6 from Ellis et al., 2013: pd1082Tg: Sequence Information Other Construct Pages ... Tīmeklis抑制/过表达hdac6后,检测自噬体(lc3)与溶酶体(lamp2)的改变,并使用mrfp-gfp-lc3报告质粒检测自噬流,分析hdac6对自噬体与溶酶体融合过程的调节作用。 进一步检测HDAC6底物Cortactin、Tubulin等的表达,分析HDAC6通过对肌动蛋白网和微管蛋白作用来调节自噬过程。 blue gray wall colors

PHLPP Sensitizes Multiple Myeloma Cells to Bortezomib Through ...

Category:Engineered exosomes for targeted co-delivery of miR-21 inhibitor …

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Lamp2 gfp

Regulation of mitochondria distribution by RhoA and formins

TīmeklisLAMP2 remained bound to a G3BP1 fragment devoid of the RGG domain ( Figure 5 N) that mediates TSC2 interaction. Thus, G3BP1 binds to TSC2 via its C-terminal RGG domain and to LAMP2 via its N ... Tīmeklis2015. gada 11. dec. · Lamp-GFP and mtKeima adenovirus were generated using the ViraPower Adenoviral Expression System (Invitrogen) according to the …

Lamp2 gfp

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TīmeklisNational Center for Biotechnology Information Tīmeklis欢迎来到赫澎(上海)生物科技有限公司公司网站,让我们从这里走进科研。赫澎生物是一家生物制剂,精细化工,基因科学等产品销售的企业。坐落在金山区,并设有储存。 赫澎(上海)生物科技有限公司“赫澎”,主要产品包括生物试剂,标准品,培养基,elisa试剂盒,多肽合成,仪器仪表等 ...

TīmeklisWT or DKO cells were first transfected with LC3-GFP (24 hours) and then nutrient-depleted for 15 hours (B) or for the indicated period (C), followed by quantitation of the frequency of cells with ... Tīmeklisリソソームは膜結合小器官で、生体分子の分解、アポトーシス、細胞シグナル伝達、代謝、および細胞膜修復などのいくつかの細胞プロセスに関与しています。. リソソームマーカーはリソソームの同定および追跡に効果的なツールです。. LysoTrackerマーカー ...

TīmeklisA. The quantification of the colocalization between a lysosomal protein such as LAMP2 and LC3 could be used via a standard immunofluorescence protocol (fixation using …

TīmeklisLAMP1/RE72002 Species D. melanogaster (fly) Insert Size (bp) 954 Promoter Ac5 Tag / Fusion Protein EGFP (C terminal on insert) Cloning Information Cloning method …

Tīmeklis2024. gada 25. marts · ( A) Schematic representation of LAMP2 genomic sequence. There are three isoforms of LAMP2 (A, B, and C) originated by the alternative … blue gray wall paint colorTīmeklis2016. gada 24. sept. · Full length GFP-LAMP2 protein migrated at ~ 140 kD and its abundance was not altered. after 4 hr cycloheximide treatment, consistent with its long half life in cultured cells (panel B). Simi- blue gray white rugTīmeklisWe now describe msGFP2, which retains monomeric superfolder properties while being as photostable as EGFP. msGFP2 contains modified N- and C-terminal peptides that … blue gray wall paintTīmeklisMoreover, GFP fluorescence in autolysosomes is attenuated due to degradation and acid quenching.12 Thus, GFP-LC3 is a good marker of early autophagic events, but it is unable to detect auto - phagy at later stages or over long time intervals. To circumvent this problem, Kimura et al., have used monomeric red fluores- freeload streamTīmeklis2016. gada 2. maijs · (A) The average fluorescence intensity of MELOPS was determined for MNT-1 cells overexpressing LAMP2-GFP, as a control (n = 31 cells), … blue gray wall to wall carpetTīmeklis(B) Immunoprecipitation confirmed the interactions between PHLPP and LAMP2 in U266 cells; (C) EGFP-PHLPP was expressed in U266 cells for 48 hrs and loaded with lysotracker-Red DND-99 for 30 mins at 37°C. Cells … bluegray wineTīmeklisLAMP2 is one of the two major lysosomal membrane proteins, and the increase in its expression partially explains the increase in the volume of primary lysosomes at … blue gray wedding dress