WebbHisTrap ™ HP 1 mL and 5 mL columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose ® High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications. WebbColumn material Polypropylene barrel, polyethylene frits Medium Ni Sepharose High Performance Average bead size 34 µm Protein binding capacity1 Approx. 750 µg …

HisTrap™ excel Columns, Cytiva VWR

WebbHisTrap™ HP is a ready to use HiTrap™ column, prepacked with precharged Ni Sepharose™ High Performance . This prepacked column is ideal for preparative … WebbPurify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High … homes for sale waterloo on

Cytiva HisTrap HP, 100 Columns: Protein Purification Protein …

WebbHisTrap HP, 100 Columns—Prepacked columns designed for simple, high-resolution purification of histidine-tagged proteins by IMAC. Shop Cytiva HisTrap … WebbWash with 5 column volumes of start buffer, at 1 ml/min (HiTrap 1 ml), 5 ml/min (HiTrap 5 ml), 3 ml/min (HiLoad 20 ml), 8 ml/min (HiLoad 53 ml) or at 50 cm/h for Sepharose High Performance packed in larger columns. Wash with 5 column volumes of elution buffer, same flow as step 2. Wash with 5 column volumes of start buffer, same flow as step 2. WebbS: Methyl sulfonate (strong cation exchanger) Mean particle diameter1~ 15 μm pH stability, operational2 pH stability, CIP3 pH ligand fully charged Entire operational pH range Maximum operating pressure4 1 mL column: 1.5 MPa (15 bar, 218 psi) 6 mL column: 0.6 MPa (6 bar, 87 psi Maximum operating flow rate4 homes for sale waterfront brick nj