Flanking sequence tag
The 5′ flanking region is a region of DNA that is adjacent to the 5′ end of the gene. The 5′ flanking region contains the promoter, and may contain enhancers or other protein binding sites. It is the region of DNA that is not transcribed into RNA. Not to be confused with the 5′ untranslated region, this region is not transcribed into RNA or translated into a functional protein. These regions primarily function in th… WebMay 1, 2013 · A total of 246 566 flanking sequence tags from rice mutant libraries with T-DNA, Ds / dSpm, or Tos17 insertion have been collected and analyzed. The results show that, among 211 470 unique hits, inserts located in the genic region account for 68.16%, and 60.49% of nuclear genes contain at least one insertion.
Flanking sequence tag
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WebDec 21, 2006 · The flanking sequence tag for each T-DNA insertion has been deposited in public databases, and seed for each line is currently available from the Arabidopsis Biological Resource Center. The pDsLox vector used to create this new population contains a Ds transposon and Cre/Lox recombination sites. Each WiscDsLox line therefore has … WebJun 26, 2012 · This “Flanking Sequence Tag” (FST) identifies the genomic location of the insertion, its orientation and, if both borders are retrieved, the length of any deletion that …
WebT-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics. The GABI-Kat population of T-DNA mutagenized Arabidopsis thaliana lines … WebThe 5′ flanking region of the human GR was first isolated and characterized by Zong et al. (57). A genomic clone of about 3.0 kb was isolated and sequenced. Computer analysis …
WebJan 28, 2024 · Protein legacy names are given in parentheses. PAM stands for protospacer adjacent motif, PFS stands for protospacer flanking site, and 3’ flank stands for 3’ flanking sequence of the protospacer that is non-complementary to the 5’-tag of crRNA; each of which are used for self vs. non-self recognition. Nucleic acids are not to scale with ... Webflanking sequence The untranscribed regions at the 5′ or 3′ ends of a transcribed gene. Source for information on flanking sequence: A Dictionary of Ecology dictionary. …
WebFlanking Sequence Tag: All FSTs: Only T-DNA: Only Tos17: Only Ac/Ds: Nothing: Region: All: Promoter: Gene: 3' UTR : Orientation: All: Reverse: Forward : Output: FASTA file: 1000 bp of sequence preceding the 5' end of each transcription unit. Gene sequences from TAIR10 release, including UTRs and introns. CDS sequences from TAIR10 release. …
WebDec 31, 2024 · GABI-Kat: generation of flanking sequence tags (FSTs) from T-DNA mutagenised A. thaliana plants accession Columbia) ... GenBank accession number of the FST or by BLAST with a sequence. Please note that the AGI gene code must be present in Araport11. The initial PI's for GABI-Kat were: Bernd Weisshaar, Koen Dekker, Bernd … ウェルズファーゴ 配当月Web発現配列タグ(はつげんはいれつタグ、英: expressed sequence tag 、略称: EST)とはcDNA配列の一部からなる短い断片であり 、遺伝学において遺伝子の転写産物の同定 … painel dizuWebOffer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days. Largest commercial ORF clones database, 70,000 next-day … ウェルスプリング 珠洲WebPrimers contain flanking sequences directing the tagging cassette to recombine into the gene end, creating a fused protein. Templates used for C’ tags should encode a stop codon at the end of the tag’s sequence, and a terminator for transcription termination before the selection cassette. painel divisor pretoWebMar 4, 2015 · The sequence analysis shows that these two T-DNAs insert the AT2G11740 gene in a head-to-head orientation. Therefore, we obtained two flanking sequences by using nested left-border specific primers (Figure 3(A)). On the contrary, no flanking sequences were amplified by using right-border primers. ウェルスプリングジャパンWebJun 26, 2012 · This “Flanking Sequence Tag” (FST) identifies the genomic location of the insertion, its orientation and, if both borders are retrieved, the length of any deletion that … ウェルスマネジメント ホテルWebJul 22, 2003 · Abstract. Summary: GABI-Kat SimpleSearch is a database of flanking sequence tags (FSTs) of T-DNA mutagenized Arabidopsis thaliana lines that were generated by the GABI-Kat project. Sequences flanking the T-DNA insertion sites were aligned to the A.thaliana genome sequence, annotated with information about the FST, … ウェルズファーゴ銀行