Determination of aconitase activity
WebTotal aconitase activity can also be measured in whole cell lysates, for example, 143B osteosarcoma lysate shown below (Figure 3). Note – the aconitase activity measured in … WebNov 11, 2003 · Determination of Aconitase Protein Levels and Activity in the Tomato Genotypes Having demonstrated that leaves of the Aco-1 plants exhibited lower …
Determination of aconitase activity
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Web0.5M sorbitol at the concentration of 200mg/ml. Aconitase activity was determined by increase in absorbance at 340nm in the presence of 5mM citrate, 0.25mM NADP, 4mM MgCl 2, 10unit/ml of NADP-isocitrate dehydroge-nase (Murakami et al., 2006). 2.3. Determination of hydrogen peroxide and superoxide anion Determination of rosmarinic … WebDetermination of Aconitase Activity—For the determination of the aconitase activity, cells from the CGXII main culture (∼25 ml) were harvested with ∼25 g of crushed ice …
WebBuy Sodium monofluoroacetate, 99% (CAS No. 62-74-8) from Smolecule. Molecular Formula: C2H2FO2.Na C2H2FO2.Na CH2FCOONa C2H2FNaO2. Molecular Weight: 100.02 g/mol. Introduction Sodium monofluoroacetate, also known as 1080, is a highly toxic chemical compound that has been categorized as a Schedule 7 poison in Australia and … WebAconitase activity determined after 15 hours incubation at 5', pH 7.4. effect. Phosphate thus competed with the protein for the iron in the system, as well as prevented the added …
WebAconitase 2 ( ACO2) encodes the mitochondrial aconitase (ACO2), an enzyme catalyzing interconversion of citrate into isocitrate in the Krebs cycle. ACO2 mutations have been … WebJan 10, 2024 · This protocol describes the determination of aconitase activity, which can provide an indirect measurement of Lon activity in vivo. The protocol is optimized for …
WebAug 1, 2005 · Unanticipated, however, was the reduced expression of the tricarboxylic acid (TCA) cycle gene citB (encoding aconitase), resulting in the loss of aconitase activity and preventing the ...
WebDec 10, 2024 · Mitochondrial Aconitase In-Gel Enzyme Activity Assays Protein samples were separated using a non-denaturing gel, and then the gel was incubated in a coloring … headlights at o\u0027reilly 41kWebJul 9, 2004 · Aconitase, a Krebs-cycle enzyme that converts citrate to isocitrate, belongs to the family of iron-sulfur–containing dehydratases whose activities depend on an intact cubane [4Fe-4S] 2+ cluster (1, 2).The purified enzyme is highly susceptible to oxidant-induced inactivation due to release of the solvent-exposed Fe-α and formation of a [3Fe … gold pearl pickguardWebAug 29, 2024 · First, in-gel activity assays showed that the mitochondrial aconitase activity (m-aco) in FRDA patient-derived cells increased significantly after SS-31 treatment. ... Determination of ... gold pearl setWebFor quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator. Key Features. Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. gold pearls clipartWebDec 13, 2002 · Determination of activities of mitochondrial complexes. For complex activity measurements, samples of synaptosomes (1 mg) were incubated in 1 mL of standard medium for 20 min at 37°C with different concentrations of rotenone, antimycin, or cyanide, respectively. ... Aconitase activity was expressed as a function of the per cent … gold pearl rings for womenWebOct 1, 1974 · TAA is also known to play a role in the TCA cycle where it inhibits the aconitase (ACO), the enzyme converting citrate to isocitrate via cis-aconitic acid (CAA) intermediate [34] [35] [36 ... headlights at reiWebJun 1, 2015 · The effect of pH on aconitase activity was studied in 100 mM tris–HCl buffer containing 40 mM citrate or 4 mM d, l-isocitrate. Determination of K i was performed by plotting the graphs of dependence of 1/v from the concentration of inhibitor [i] at two fixed concentrations of substrate (citrate) (Dixon, 1953). gold pearl ring settings